ROX
™
OPTICALDISSOLVEDOXYGENCALIBRATIONTIPS
ROX FACTS
• The ROX Optical Dissolved Oxygen sensor does not require any special sonde setup or burn-in.
• Calibration data is stored in the ROX so it can be calibrated in one sonde and then used in another
without recalibrating the probe in the new sonde.
• Calibration data is automatically transferred to the host sonde as soon as the sonde powers up the
ROX sensor.
• The ROX has excellent long term stability and will achieve ± 1% accuracy when calibrated in a
(temperature-stable) saturated water or saturated air environment.
• Field DO calibrations should be avoided!
• Remove the wiper when the sensor is going to be exposed to sodium sulfite (zero DO test) or
Formazin-based turbidity standards.
• The ROX sensor must remain hydrated at all times! See Appendix M in the 6-Series manual for
additional information.
CALIBRATION METHOD – ONE-POINT “SATURATED AIR”
1. Dry the temperature sensor and the ROX membrane to remove any water droplets.
2. Put 1 inch of water in the calibration cup and thread onto the endcap ½ thread.
Do not fully thread the calibration cup onto the endcap!
3. Allow the sonde to sit in the calibration cup for 15 minutes to ensure that the air is 100%
saturated and that the temperature is stable.
4. Select ODO% and enter the barometric pressure in mmHg (inches x 25.4) and press Enter. The
sonde will do a 4 second count down and then say “Press Enter when the readings are stable”.
Wait at least 30 seconds before you press the Enter button.
Note: the sonde will only read 100% saturation if the barometric pressure is 760 mmHg. To
determine the actual DO in %, divide the barometer reading by 760 and then multiply that
number by 100.
Example: 750 ÷ 760 = 0.9868 x100 = 98.68%
Check the ODO Gain in the Advanced Menu. An acceptable range is 0.85 to 1.15, NOTE:
Maximum range before getting error message is 1 ± .25.
CALIBRATION METHOD – ONE-POINT “SATURATED WATER”
1. Using a medium-size aquarium air pump and air stone, sparge a five-gallon bucket of tap water
for 1 hour.
2. Place the assembled sonde in the bucket and allow the sonde to equilibrate to the water
temperature for 5 minutes.
3. Select ODO% and enter the barometric pressure in mmHg (inches x 25.4) and press Enter. The
sonde will do a four-second countdown and then say “Press Enter when the readings are
stable”. Wait at least 30 seconds before you press the Enter button.
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